Application
Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 μg/mLApplication
Immunofluorescence of BAG-1 in Human Lymph Node cells with BAG-1 antibody at 20 μg/mL.Application
Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BAG-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAG-1 Antibody: Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.1) Takayama S, Sato T, Kraweski K, et al. Cloning and functional analysis of BAG-1: a novel Bcl2-binding protein with anti-cell death activity. Cell 1995; 80:279-84. |
| Immunogen | BAG-1 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human BAG-1. The immunogen is located within the first 50 amino acids of BAG-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAG1 |
|---|---|
| Alternative Names | BAG-1 Antibody: HAP BAG-1 RAP46 HAP BAG family molecular chaperone regulator 1 Bcl-2-associated athanogene 1 |
| Sequence Similarities | 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 μg/mL
Application
Immunofluorescence of BAG-1 in Human Lymph Node cells with BAG-1 antibody at 20 μg/mL.
Application
Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | BAG-1 antibody can be used for the detection of BAG-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAG-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAG-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
