Application
Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of Bak in L1210 cells with Bak antibody at 2 μg/mL.| Product Name | Bak Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bak Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria. It is thought to share significant functional homology with Bax, another pro-apoptotic Bcl-2 family member, as disruption of bak or bax has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking bak and bax show decreased apoptotic capability.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bak antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of human Bak. The immunogen is located within amino acids 30 - 80 of Bak. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Bak antibody can be used for detection of Bak by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAK1 |
|---|---|
| Alternative Names | Bak Antibody: BAK CDN1 BCL2L7 BAK-LIKE BAK Apoptosis regulator BAK Bcl2-L-7 |
| Sequence Similarities | 2) Cat. No. 17-204 - L1210 Cell Slide |
Application
Western blot analysis of Bak in L1210 cell lysates with Bak antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of Bak in L1210 cells with Bak antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1284 - L1210 Cell Lysate |
|---|---|
| Application Notes | Bak antibody can be used for detection of Bak by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bak antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bak Antibody is supplied in PBS containing 0.02% sodium azide. |
