Application
Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 μg/mL.Application
Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 μg/mL.| Product Name | BANP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BANP Antibody: BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-beta signaling. Furthermore, BANP can modulate NF-κB transactivation and can inhibit tumorigenesis by regulating NF-κB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix.1) Birot A, Duret L, Bartholin L, et al. Identification and molecular analysis of BANP. Gene2000; 253:189-96. |
| Immunogen | BANP antibody was raised against a 14 amino acid synthetic peptide near the center of human BANP. The immunogen is located within amino acids 300 - 350 of BANP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BANP antibody can be used for detection of BANP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BANP |
|---|---|
| Alternative Names | BANP Antibody: BEND1 SMAR1 SMARBP1 BEND1 Protein BANP BEN domain-containing protein 1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Entrez Gene | 54971 |
|---|---|
| Protein Accession | NP_001167014 |
Application
Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 μg/mL.
Application
Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | BANP antibody can be used for detection of BANP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BANP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BANP Antibody is supplied in PBS containing 0.02% sodium azide. |
