Application
Immunohistochemistry of BAPX1 in mouse brain tissue with BAPX1 antibody at 5 μg/mL.Application
Immunofluorescence of BAPX1 in mouse brain tissue with BAPX1 antibody at 20 μg/mL.Application
Western blot analysis of BAPX1 in human brain tissue lysate with BAPX1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | BAPX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAPX1 Antibody: BAPX1 is the mammalian homolog of the Drosophila bagpipe homeobox gene and is expressed in the splanchnic mesoderm and embryonic skeleton. It is one of the earliest developmental markers for the sclerotome portion of the somite and the gut mesentery. BAPX1 is required for normal skeletal development; homozygous inactivating mutations in the BAPX1 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia (SMMD). It has also been suggested to play a role in the proper development of the mammalian gut and is required for distal stomach development as part of a BARX1-dependent pathway.1) Tribioli C, Frasch M, and Lufkin T. Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe homeobox gene is expressed in splanchnic mesoderm and the embryonic skeleton. Mech. Dev. 1997; 65:145-62. |
| Immunogen | BAPX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human BAPX1. The immunogen is located within amino acids 180 - 230 of BAPX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BAPX1 antibody can be used for detection of BAPX1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX3-2 |
|---|---|
| Alternative Names | BAPX1 Antibody: SMMD BAPX1 NKX3B NKX3.2 Bagpipe homeobox protein homolog 1 |
| Tissue Specificity | BAPX1 antibody is predicted not to cross-react with other NKX homeobox proteins. |
Application
Immunohistochemistry of BAPX1 in mouse brain tissue with BAPX1 antibody at 5 μg/mL.
Application
Immunofluorescence of BAPX1 in mouse brain tissue with BAPX1 antibody at 20 μg/mL.
Application
Western blot analysis of BAPX1 in human brain tissue lysate with BAPX1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | BAPX1 antibody can be used for detection of BAPX1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAPX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAPX1 Antibody is supplied in PBS containing 0.02% sodium azide. |
