Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in A431,Hela,Jurkat cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | BAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. It has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. It can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. It can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4.1) Choudhary C., et.al., Science 325:834-840(2009). |
| Immunogen | This BAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from the C-terminal region of human BAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDX39B |
|---|---|
| Alternative Names | Spliceosome RNA helicase DDX39B 56 kDa U2AF65-associated protein ATP-dependent RNA helicase p47 DEAD box protein UAP56 HLA-B-associated transcript 1 protein DDX39B BAT1 UAP56 |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in A431,Hela,Jurkat cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
