Application
Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.Application
Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.| Product Name | BATF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BATF Antibody: BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.1) Dorsey MJ, Tae HJ, Sollenberger KG, et al. B-ATF: a novel human bZIP protein that associates with members of the AP-1 transcription factor family. Oncogene 1995; 11:2255-65. |
| Immunogen | BATF antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BATF. The immunogen is located within the first 50 amino acids of BATF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BATF |
|---|---|
| Alternative Names | BATF Antibody: SFA2 B-ATF BATF1 SFA-2 Basic leucine zipper transcriptional factor ATF-like B-cell-activating transcription factor |
| Molecular Weight(MW) | 14 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | Multiple isoforms of BATF are known to exist. Despite its predicted molecular weight, BATF often migrates at a higher than expected molecular weight. |
Application
Immunofluorescence of BATF in human spleen tissue with BATF antibody at 20 μg/mL.
Application
Western blot analysis of BATF in rat spleen tissue lysate with BATF antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | BATF antibody can be used for detection of BATF by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BATF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BATF Antibody is supplied in PBS containing 0.02% sodium azide. |
