Application
Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.Application
Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 μg/mL.Application
Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.| Product Name | Bax Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bax Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bax, a pro-apoptotic Bcl-2 family member, is a cytosolic protein that changes conformation and translocates to the mitochondria following apoptotic stimuli. It is thought to share significant functional homology with Bak, another pro-apoptotic Bcl-2 family member, as disruption of bax or bak has little effect on cell death, but mice lacking both genes display multiple developmental defects and cells lacking both show decreased apoptotic capability.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bax antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Bax. The immunogen is located within the first 50 amino acids of Bax. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIPWB |
| Bax antibody can be used for detection of Bax by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAX |
|---|---|
| Alternative Names | Bax Antibody: BCL2L4 BCL2L4 Apoptosis regulator BAX Bcl-2-like protein 4 Bcl2-L-4 |
| Sequence Similarities | 2) Cat. No. 17-009 - HL-60 Cell Slide |
Application
Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.
Application
Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 μg/mL.
Application
Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1209 - HL60 Cell Lysate |
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| Application Notes | Bax antibody can be used for detection of Bax by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bax antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bax Antibody is supplied in PBS containing 0.02% sodium azide. |
