Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human, Mouse, Rat.Application
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)).Application
Antibody used in IHC on Human kidney.| Product Name | BAX Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.1) Yamaguchi, H., et al., (2004) J. Biol. Chem. 279 (38), 39431-39437. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BAX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| BAX antibody can be used for detection of BAX by ELISA at 1:62500. BAX antibody can be used for detection of BAX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BAX |
|---|---|
| Alternative Names | BAX BCL2L4 |
| Molecular Weight(MW) | 21 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human, Mouse, Rat.
Application
Antibody used in WB on Human NT-2 and Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)).
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | BAX antibody can be used for detection of BAX by ELISA at 1:62500. BAX antibody can be used for detection of BAX by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BAX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
