Application
To detect hBCA-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BCA-1 (XP-5106Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBCA-1.| Product Name | BCA-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | B lymphocyte chemoattractant is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBCA 1 (human B Cell-Attracting Chemokine-1). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| ELISA: To detect hBCA 1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBCA 1. Western Blot: To detect hBCA 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBCA 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL13 |
|---|---|
| Alternative Names | BLC BCA1 ANGIE BCA-1 BLR1L ANGIE2 SCYB13 BLC C-X-C motif chemokine 13 Angie |
Application
To detect hBCA-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BCA-1 (XP-5106Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBCA-1.| Application Notes | ELISA: To detect hBCA 1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBCA 1. Western Blot: To detect hBCA 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBCA 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Form | Liquid |
|---|---|
| Storage Instructions | BCA-1 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
