Application
Immunohistochemistry of BCAS2 in human breast carcinoma with BCAS2 antibody at 5 μg/mL.Application
Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 μg/mL.Application
Western blot analysis of BCAS2 in MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | BCAS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCAS2 Antibody: BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.1) Nagasaki K, Maass N, Manabe T, et al. Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. Cancer Lett.1999; 140:219-26. |
| Immunogen | BCAS2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| BCAS2 antibody can be used for detection of BCAS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAS2 |
|---|---|
| Alternative Names | BCAS2 Antibody: DAM1 SPF27 Snt309 DAM1 Pre-mRNA-splicing factor SPF27 Breast carcinoma-amplified sequence 2 |
Application
Immunohistochemistry of BCAS2 in human breast carcinoma with BCAS2 antibody at 5 μg/mL.
Application
Immunofluorescence of BCAS2 in human breast carcinoma tissue with BCAS2 antibody at 20 μg/mL.
Application
Western blot analysis of BCAS2 in MCF7 cell lysate with BCAS2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | BCAS2 antibody can be used for detection of BCAS2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCAS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BCAS2 Antibody is supplied in PBS containing 0.02% sodium azide. |
