Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-56). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-56).Application
Western blot analysis of lysed extracts from HeLa, A2780 and 293 cells using BCL-2 (Ab-56).| Product Name | BCL 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).1) Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S. J. and Cidlowski, J. A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST, et al. (2002) FASEB J Jun; 16 (8): 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478. |
| Immunogen | BCL-2 (Ab-56) antibody was raised against a peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2 |
|---|---|
| Alternative Names | Bcl-2 PPP1R50 OIP3 PK2 PK3 PKM2 BCL2 |
| Molecular Weight(MW) | 26 kDa |
| Tissue Specificity | This antibody detects endogenous level of total BCL-2 protein. |
| Entrez Gene | 596 |
|---|---|
| Protein Accession | NP_000624.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-56). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-56).
Application
Western blot analysis of lysed extracts from HeLa, A2780 and 293 cells using BCL-2 (Ab-56).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
