Application
Western blot analysis of lysed extracts from MCF cells using BCL-2 (Ab-70).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-70).| Product Name | BCL 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCL-2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.1) Muller IM, et al. (2005) Mol Pharmacol. Jin Z, Gao F, et al. J Biol Chem 2004 Sep 17; 279 (38): 40209-19. Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3 (3): 327-34. Huang ST, et al. (2002) FASEB J; 16 (8): 825-32. Yamamoto K, et al. (1999) Mol Cell Biol; 19 (12): 8469-78. |
| Immunogen | BCL-2 (Ab-70) antibody was raised against a peptide sequence around aa.68~72 (R-T-S-P-L) derived from Human BCL-2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2 |
|---|---|
| Alternative Names | Bcl-2 PPP1R50 PKR PRKR BCL2 |
| Molecular Weight(MW) | 26 kDa |
| Tissue Specificity | This antibody detects endogenous level of total BCL-2 protein. |
| Entrez Gene | 596 |
|---|---|
| Protein Accession | NP_000624.2 |
Application
Western blot analysis of lysed extracts from MCF cells using BCL-2 (Ab-70).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2 (Ab-70). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2 (Ab-70).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
