Application
Immunofluorescence of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 20 μg/mL.Application
Immunohistochemical staining of human kidney using Bcl-2 antibody at 5 μg/mL.Application
Immunohistochemistry of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 5 μg/mL.Application
Western blot analysis of Bcl-2 in A-20 cell lysate with Bcl-2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of Bcl-2 in Human Kidney cells with Bcl-2 antibody at 10 μg/mL.| Product Name | Bcl-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N terminus of human Bcl-2. The immunogen is located within the first 50 amino acids of Bcl-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2 |
|---|---|
| Alternative Names | Bcl-2 Antibody: Bcl-2 PPP1R50 Apoptosis regulator Bcl-2 |
| Molecular Weight(MW) | Predicted: 23, 26 kDa Observed: 26 kDa |
Application
Immunofluorescence of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 20 μg/mL.
Application
Immunohistochemical staining of human kidney using Bcl-2 antibody at 5 μg/mL.
Application
Immunohistochemistry of Bcl 2 in mouse brain tissue with Bcl 2 Antibodyat 5 μg/mL.
Application
Western blot analysis of Bcl-2 in A-20 cell lysate with Bcl-2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of Bcl-2 in Human Kidney cells with Bcl-2 antibody at 10 μg/mL.| Positive Control | A20 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-2antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
