Application
Western blot analysis of Bcl-2 in (A) Daudi and (B) A-20 cell lysates with Bcl-2 antibody at 2 μg/mL.Application
Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 μg/mL.| Product Name | Bcl-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bcl-2 antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bcl-2. The immunogen is located within amino acids 80 - 130 of Bcl-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2 |
|---|---|
| Alternative Names | Bcl-2 Antibody: Bcl-2 PPP1R50 Apoptosis regulator Bcl-2 |
| Molecular Weight(MW) | Predicted: 23, 26 kDa Observed: 22 kDa |
| Sequence Similarities | 3) Cat. No. 17-208 - A-20 Cell Slide |
Application
Western blot analysis of Bcl-2 in (A) Daudi and (B) A-20 cell lysates with Bcl-2 antibody at 2 μg/mL.
Application
Immunocytochemistry of Bcl-2 in A20 cells with Bcl-2 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
