Application
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL.Application
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bcl-B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-B Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.1) Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607 |
| Immunogen | Bcl-B antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human Bcl-B. The immunogen is located within amino acids 90 - 140 of Bcl-B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L10 |
|---|---|
| Alternative Names | Bcl-B Antibody: Boo Diva BCL-B BCLB Bcl-2-like protein 10 Anti-apoptotic protein NrH Bcl2-L-10 |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 26 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/mL.
Application
Western blot analysis of Bcl-B in Jurkat cell lysate with Bcl-B antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-B Antibody is supplied in PBS containing 0.02% sodium azide. |
