Application
Western blot analysis of Bcl-xL in A549 cell lysates with Bcl-xL antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bcl-xL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-xL Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-xL is a member of the Bcl-2 family of proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-xL is an anti-apoptotic mitochondrial protein related to Bcl-w and the major transcript of the bcl-x gene. Its high expression in tumors is correlated with advanced disease and poor prognosis. Bcl-xL expression level increases in response to several stimuli such as ionizing radiation and treatment with chemotherapeutic agents.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bcl-xL antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Bcl-xL. The immunogen is located within the last 50 amino acids of Bcl-xL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Bcl-xL antibody can be used for detection of Bcl-xL by Western blot at 1 to 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | BCL2L1 |
|---|---|
| Alternative Names | Bcl-xL Antibody: BCLX BCL2L BCLXL BCLXS Bcl-X bcl-xL bcl-xS PPP1R52 BCL-XL/S BCLX Bcl-2-like protein 1 Apoptosis regulator Bcl-X Bcl2-L-1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
Application
Western blot analysis of Bcl-xL in A549 cell lysates with Bcl-xL antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | Bcl-xL antibody can be used for detection of Bcl-xL by Western blot at 1 to 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-xL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-xL Antibody is supplied in PBS containing 0.02% sodium azide. |
