Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Product Name | BCL2L1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCL2L1 encodes a protein which belongs to the BCL-2 protein family. The proteins encoded by BCL2L1 are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis.The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.1) Allikmets, R., (2006) J. Mol. Biol. 356 (2), 367-381. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BCL2L1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BCL2L1 antibody can be used for detection of BCL2L1 by ELISA at 1:312500. BCL2L1 antibody can be used for detection of BCL2L1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL2L1 |
|---|---|
| Alternative Names | BCL2L1 BCL-XL/S BCL2L BCLX Bcl-X DKFZp781P2092 bcl-xL bcl-xS BCLXL BCLXS PPP1R52 |
| Molecular Weight(MW) | 26 kDa, 19 kDa |
Application
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | BCL2L1 antibody can be used for detection of BCL2L1 by ELISA at 1:312500. BCL2L1 antibody can be used for detection of BCL2L1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BCL2L1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
