Application
Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr (Phospho-Tyr177).Application
Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using Bcr (Phospho-Tyr177).| Product Name | Bcr (phospho Tyr177) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.1) Sattler M, et al. Mol Cell Biol. 1999 Nov; 19 (11): 7473-7480. Li S et al. EMBO J. 2001 Dec 3; 20 (23): 6793-6804. Million RP, et, al. Mol Cell Biol. 2004 Jun; 24 (11): 4685-4695. Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 – 30916. |
| Immunogen | Bcr (Phospho-Tyr177) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y (p) -V-N) derived from Human Bcr. |
| Modification | p-Tyr177 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCR |
|---|---|
| Alternative Names | ALL CML PHL BCR1 D22S11 D22S662 CFL BCR BCR protein |
| Molecular Weight(MW) | 210 kDa |
| Tissue Specificity | This antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177. |
| Entrez Gene | 613 |
|---|---|
| Protein Accession | NP_004318.3 |
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tumor tissue using Bcr (Phospho-Tyr177).
Application
Western blot analysis of lysed extracts from K562 cells untreated or treated with H2O2 using Bcr (Phospho-Tyr177).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
