Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using β-Catenin (Ab-33). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using β-Catenin (Ab-33).Application
Western blot analysis of lysed extracts from HeLa and HT29 cells using β-Catenin (Ab-33).| Product Name | beta Catenin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.1) Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95 (8): 4374-4379Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100 (18): 10193-10200Okamura H, et al. (2004) Mol Cell Biol; 24 (10): 4184-4195Xing Y, et al. (2003) Genes Dev; 17 (22): 2753-2764Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96 (9): 4947-4952 |
| Immunogen | beta-Catenin (Ab-33) antibody was raised against a peptide sequence around aa.31~35 (L-D-S-G-I) derived from Human β-Catenin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTNNB1 |
|---|---|
| Alternative Names | CTNNB MRD19 armadillo EPN CTNNB1 CATNB CTNB1 CTNNB |
| Molecular Weight(MW) | 92 kDa |
| Tissue Specificity | This antibody detects endogenous level of total β-Catenin protein. |
| Entrez Gene | 1499 |
|---|---|
| Protein Accession | NP_001091679.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using β-Catenin (Ab-33). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using β-Catenin (Ab-33).
Application
Western blot analysis of lysed extracts from HeLa and HT29 cells using β-Catenin (Ab-33).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
