Application
Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k beta-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan beta-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3beta knocked down by SiRNA.| Product Name | Beta-Catenin (phospho Ser33 / Ser37)Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Beta-catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of beta-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3beta (GSK3beta) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of beta-catenin. Mutations in components of this phosphorylation regulated process that prevent beta-catenin hyperphosphorylation by GSK3beta are strongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).1) Ding Y, Dale, T (2002) Wnt signal transduction: kinase cogs in a nano-machine? Trends Biochem Sci 27:327-329. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser33, 37 of human beta-catenin. |
| Modification | p-Ser33 / Ser37 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. | |
| Species Reactivity | HumanMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTNNB1 |
|---|---|
| Alternative Names | CTNNB MRD19 armadillo CTNNB |
| Molecular Weight(MW) | 83 |
| Sequence Similarities | ORF Names: OK/SW-cl.35, PRO2286, Beta-catenin, |
Application
Western blot of human embryonic kidney cell (HEK) lysate showing specific immunolabeling of the ~83k beta-catenin phosphorylated at Ser33 and Ser37 (Upper Panel) and a pan beta-catenin (Lower Panel). Control is a standard HEK cell lysate. GSK3 is a HEK cell lysate with glycogen synthase kinase 3beta knocked down by SiRNA.| Application Notes | The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will react with mouse, rat and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Beta-Catenin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
