Application
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugateApplication
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in RD cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Product Name | BHLH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.1) Honma, S., et al. Nature 419(6909):841-844(2002) Garriga-Canut, M., et al. J. Biol. Chem. 276(18):14821-14828(2001) Fujimoto, K., et al. Biochem. Biophys. Res. Commun. 280(1):164-171(2001) Grottke, C., et al. Int. J. Cancer 88(4):535-546(2000) |
| Immunogen | This BHLH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BHLHE41 |
|---|---|
| Alternative Names | Class E basic helix-loop-helix protein 41 bHLHe41 Class B basic helix-loop-helix protein 3 bHLHb3 Differentially expressed in chondrocytes protein 2 hDEC2 Enhancer-of-split and hairy-related protein 1 SHARP-1 BHLHE41 BHLHB3 DEC2 SHARP1 |
| Molecular Weight(MW) | 50 kDa |
Application
Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugate
Application
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in RD cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
