Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
BIN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in L929 cell line lysates (35ug/lane).| Product Name | BIN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.1) Biffi, A., et al. Arch. Neurol. 67(6):677-685(2010) Seshadri, S., et al. JAMA 303(18):1832-1840(2010) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) Claeys, K.G., et al. Neurology 74(6):519-521(2010) Hong, T.T., et al. PLoS Biol.(2), E1000312 (2010) : |
| Immunogen | This BIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-389 amino acids from the Central region of human BIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BIN1 |
|---|---|
| Alternative Names | Myc box-dependent-interacting protein 1 Amphiphysin II Amphiphysin-like protein Box-dependent myc-interacting protein 1 Bridging integrator 1 BIN1 AMPHL |
| Molecular Weight(MW) | 65 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
BIN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in L929 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
