Application
Immunohistochemistry of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 5 μg/mL.Application
Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1μg/mL.| Product Name | Blimp-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Blimp-1 Antibody: Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells. Blimp-1 exists as at least two different isoforms.1) Angelin-Duclos C, Cattoretti G, Lin K-I, et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression. J. Immunol. 2000; 165:5462-71. |
| Immunogen | Blimp-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Blimp-1. The immunogen is located within amino acids 100 - 150 of Blimp-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDM1 |
|---|---|
| Alternative Names | Blimp-1 Antibody: BLIMP1 PRDI-BF1 BLIMP1 PR domain zinc finger protein 1 BLIMP-1 |
Application
Immunohistochemistry of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 5 μg/mL.
Application
Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Blimp-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Blimp-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM272530