Application
Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1μg/mL.Application
Immunofluorescence of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 20 μg/mL.| Product Name | Blimp-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Blimp-1 Antibody: Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells.1) Turner CAJ, Mack DH, and Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B-lymphocytes into immunoglobulin-secreting cells. Cell 1994; 77:297-306. |
| Immunogen | Blimp-1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Blimp-1. The immunogen is located within the last 50 amino acids of Blimp-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDM1 |
|---|---|
| Alternative Names | Blimp-1 Antibody: BLIMP1 PRDI-BF1 BLIMP1 PR domain zinc finger protein 1 BLIMP-1 |
Application
Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1μg/mL.
Application
Immunofluorescence of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Blimp-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Blimp-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM272531