Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | BLK (phospho Tyr501) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | p55-BLK, EC 2.7.10.2, kinase Blk, B lymphocyte kinase, Tyrosine-protein kinase BLK |
| Product description | Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor.1) Islam K.B., J. Immunol. 154:1265-1272(1995). |
| Immunogen | BLK (phospho Tyr501) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 501 (R-Q-Y (p)-E-L) derived from Human BLK. |
| Modification | p-Tyr501 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BLK |
|---|---|
| Alternative Names | p55-BLK EC 2.7.10.2 kinase Blk B lymphocyte kinase Tyrosine-protein kinase BLK |
| Molecular Weight(MW) | 57 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BLK only when phosphorylated at tyrosine 501. |
| Entrez Gene | 640 |
|---|---|
| Protein Accession | NP_001706.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BLK (Phospho-Tyr501) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
