Application
Western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).Application
Fluorescent image of Hela cells stained with BLMH Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Product Name | BLMH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH |
| Product description | The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).1) Barrow I.K.-P., et al. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases. |
| Immunogen | This BLMH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the Central region of human BLMH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BLMH |
|---|---|
| Alternative Names | Bleomycin hydrolase BH BLM hydrolase BMH BLMH |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in CEM,HL-60 cell line lysates (35ug/lane).
Application
Fluorescent image of Hela cells stained with BLMH Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Application Notes | For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
