Application
Immunocytochemistry of BMP15 in 3T3 cells with BMP15 antibody at 2.5 μg/mL.Application
Immunofluorescence of BMP15 in 3T3 cells with BMP15 antibody at 20 μg/mL.Application
Western blot analysis of BMP15 in human testis tissue lysate with BMP15 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BMP15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMP15 Antibody: ODG2, POF4, GDF9B, Bone morphogenetic protein 15, BMP-15 |
| Product description | BMP15 Antibody: BMP15, or GDF9B is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily that are involved in embryonic development and adult tissue homeostasis. BMP15 is expressed exclusively in the oocyte. It is an oocyte-specific growth/differentiation factor that may be involved in oocyte maturation and follicular development. Defects in BMP15 are the cause of ovarian dysgenesis type 2. BMP15 could be used as an oogenesis marker to track human amniotic fluid stem cells differentiation into the oocyte-like cells.1) Massagué J. The transforming growth factor-beta family. Ann. Rev. Cell. Biol. 1990; 6:597-641 |
| Immunogen | BMP15 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BMP15. The immunogen is located within amino acids 30 - 80 of BMP15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| BMP15 antibody can be used for detection of BMP15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMP15 |
|---|---|
| Alternative Names | BMP15 Antibody: ODG2 POF4 GDF9B Bone morphogenetic protein 15 BMP-15 |
| Molecular Weight(MW) | Predicted: 45 kDa Observed: 50 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | It is closely related to GDF9. |
Application
Immunocytochemistry of BMP15 in 3T3 cells with BMP15 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BMP15 in 3T3 cells with BMP15 antibody at 20 μg/mL.
Application
Western blot analysis of BMP15 in human testis tissue lysate with BMP15 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | BMP15 antibody can be used for detection of BMP15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BMP15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BMP15 Antibody is supplied in PBS containing 0.02% sodium azide. |
