Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-BMPR1A Pab in Saos-2 cell lysateApplication
Western blot analysis of lysate from rat uterus tissue lysate, using BMPR1A Antibody (E45) at 1:1000.Application
Western blot analysis of lysate from mouse kidney tissue, using BMPR1A Antibody (E45) at 1:1000.| Product Name | BMPR1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.1) Kan, L. et al. Stem Cells. January; 27(1): 150?56(2009). Waite, K.A., et al., Hum. Mol. Genet. 12(6):679-684 (2003). Zhou, X.P., et al., Am. J. Hum. Genet. 69(4):704-711 (2001). Astrom, A.K., et al., Mamm. Genome 10(3):299-302 (1999). ten Dijke, P., et al., Oncogene 8(10):2879-2887 (1993). Ide, H., et al., Cytogenet. Cell Genet. 81 (3-4), 285-286 (1998). |
| Immunogen | This BMPR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human BMPR1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | BMPR1A |
|---|---|
| Alternative Names | Bone morphogenetic protein receptor type-1A BMP type-1A receptor BMPR-1A Activin receptor-like kinase 3 ALK-3 Serine/threonine-protein kinase receptor R5 SKR5 CD292 BMPR1A ACVRLK3 ALK3 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-BMPR1A Pab in Saos-2 cell lysate
Application
Western blot analysis of lysate from rat uterus tissue lysate, using BMPR1A Antibody (E45) at 1:1000.
Application
Western blot analysis of lysate from mouse kidney tissue, using BMPR1A Antibody (E45) at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
