Application
Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 μg/mL.Application
Western blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 μg/mL.Application
Immunofluorescence of Bnip3L in Human Kidney tissue with Bnip3L antibody at 10 μg/mL.| Product Name | Bnip3L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bnip3L Antibody: NIX, BNIP3a, BNIP3A, BNIP3H, NIX, Adenovirus E1B19K-binding protein B5, NIP3L |
| Product description | Bnip3L Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.1) Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998; 21:230-5 |
| Immunogen | Bnip3L antibody was raised against a 16 amino acid peptide near the center of human Bnip3L. The immunogen is located within amino acids 60 - 110 of Bnip3L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bnip3L antibody can be used for detection of Bnip3L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BNIP3L |
|---|---|
| Alternative Names | Bnip3L Antibody: NIX BNIP3a BNIP3A BNIP3H NIX Adenovirus E1B19K-binding protein B5 NIP3L |
| Molecular Weight(MW) | Predicted: 24 kDa Observed: 36, 40 kDa |
| Sequence Similarities | 3) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least two isoforms of Bnip3L are known to exist. |
Application
Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 μg/mL.
Application
Western blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 μg/mL.
Application
Immunofluorescence of Bnip3L in Human Kidney tissue with Bnip3L antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Bnip3L antibody can be used for detection of Bnip3L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Bnip3L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bnip3L Antibody is supplied in PBS containing 0.02% sodium azide. |
