Application
PSMB1 antibody detects PSMB1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMB1 antibody diluted at 1:1000.Application
PSMB1 antibody detects PSMB1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PSMB1 antibody diluted at 1:500.Application
PSMB1 antibody detects PSMB1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PSMB1 antibody diluted at 1:500.| Product Name | PSMB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMB1The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, Chicken, Rhesus Monkey, BovineZebrafish (80%), Pig (97%), Chicken (85%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HC5 PMSB1 PSC5 proteasome (prosome macropain) subunit beta type 1 |
|---|---|
| Target | PSMB1 |
| Molecular Weight(MW) | 26 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
PSMB1 antibody detects PSMB1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMB1 antibody diluted at 1:1000.
Application
PSMB1 antibody detects PSMB1 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PSMB1 antibody diluted at 1:500.
Application
PSMB1 antibody detects PSMB1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PSMB1 antibody diluted at 1:500.| Positive Control | 293T , A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
