Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Phospho-Ser1423).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using BRCA1 (Phospho-Ser1423).| Product Name | BRCA1 (phospho Ser1423) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53, ERYF1, GF1 |
| Product description | The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation1) Kim ST, et al. (2002) Genes Dev; 16 (5): 560-570Arlt MF, et al. (2004) Mol Cell Biol; 24 (15): 6701-6709Zhang J, et al. (2004) Mol Cell Biol; 24 (2): 708-718Greenberg RA, et al. (2006) Genes Dev; 20 (1): 34-46Tibbetts RS, et al. (2000) Genes Dev; 14 (23): 2989-3002 |
| Immunogen | BRCA1 (Phospho-Ser1423) antibody was raised against a peptide sequence around phosphorylation site of serine 1423 (H-G-S (p) -Q-P) derived from Human BRCA1. |
| Modification | p-Ser1423 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA1 |
|---|---|
| Alternative Names | IRIS PSCP BRCAI BRCC1 PNCA4 RNF53 BROVCA1 PPP1R53 ERYF1 GF1 |
| Molecular Weight(MW) | 220 kDa |
| Tissue Specificity | This antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423. |
| Entrez Gene | 672 |
|---|---|
| Protein Accession | NP_009225.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1 (Phospho-Ser1423).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using BRCA1 (Phospho-Ser1423).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
