Application
Western blot analysis of extracts from 293 cells (Lane 2), using BRCA1 (Phospho-Ser1457) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | BRCA1 (phospho Ser1457) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRCA1, Breast cancer type 1 susceptibility protein, familial breast/ovarian cancer gene 1 |
| Product description | The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation1) Miki Y., Science 266:66-71(1994). |
| Immunogen | BRCA1 (phospho Ser1457) antibody was raised against a peptide sequence around phosphorylation site of Serine 1457 (L-T-S (p)-Q-K) derived from Human BRCA1. |
| Modification | p-Ser1457 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA1 |
|---|---|
| Alternative Names | BRCA1 Breast cancer type 1 susceptibility protein familial breast/ovarian cancer gene 1 |
| Molecular Weight(MW) | 220 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1457. |
| Entrez Gene | 672 |
|---|---|
| Protein Accession | NP_009225.1 |
Application
Western blot analysis of extracts from 293 cells (Lane 2), using BRCA1 (Phospho-Ser1457) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
