Application
Antibody used in IHC on Human Muscle.Application
Antibody used in WB on Human K562 at 0.5-1 ug/ml.| Product Name | BRCA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRCA1, IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53 |
| Product description | BRCA1, which functions as a tumor suppressor in human breast cancer cells, is a nuclear phosphoprotein which associates with RNA polymerase II holoenzyme. Mutations in BRCA1 are predicted to be responsible for approximately 45% of inherited breast cancer and more than 80% of inherited breast and ovarian cancer. BRCA1 may function as a transcriptional regulator, due to an amino terminal DNA-binding ring finger motif, nuclear localization signals, and an acidic carboxy terminal domain. BRCA1 is also a granin-like protein that functions as a secreted growth inhibitory protein. BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth. This function is compromised in breast cancer either by direct mutation or by alterations in gene expression.1) Mark, W.Y., et al., (2005) J. Mol. Biol. 345 (2), 275-287. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRCA1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA1 |
|---|---|
| Alternative Names | BRCA1 IRIS PSCP BRCAI BRCC1 PNCA4 RNF53 BROVCA1 PPP1R53 |
| Molecular Weight(MW) | 76 kDa, 208 kDa, 208 kDa, 208 kDa, 181 kDa, 178 kDa, 166 kDa, 203 kDa, 81 kDa, 85 kDa, 81 kDa, 7 kDa |
Application
Antibody used in IHC on Human Muscle.
Application
Antibody used in WB on Human K562 at 0.5-1 ug/ml.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRCA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose.. Final antibody concentration is 1 mg/mL. |
