Application
Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody.Application
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody at dilution of 1:200 (400x lens).Application
Immunofluorescence analysis of HeLa cell using PSMB10 antibody. Blue: DAPI for nuclear staining.| Product Name | PSMB10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMB10The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human PSMB10 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | proteasome (prosome macropain) subunit beta type 10 beta2i MECL1 LMP10 |
|---|---|
| Target | PSMB10 |
| Molecular Weight(MW) | 29 kDa (note) |
Application
Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody.
Application
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody at dilution of 1:200 (400x lens).
Application
Immunofluorescence analysis of HeLa cell using PSMB10 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
