Application
Immunohistochemistry of BRCC45 in human brain tissue with BRCC45 antibody at 2.5 μg/mL.Application
Immunofluorescence of BRCC45 in human brain tissue with BRCC45 antibody at 20 μg/mL.Application
Western blot analysis of BRCC45 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with BRCC45 antibody at 1 μg/mL.| Product Name | BRCC45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRCC45 Antibody: BRCC4, BRCC45, BRCA1-A complex subunit BRE, Brain and reproductive organ-expressed protein |
| Product description | BRCC45 Antibody: BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.1) Li L, Yoo H, Beckker FF, et al. Identification of a brain- and reproductive-organs-specific gene responsive to DNA damage and retinoic expression. Biochim. Biophys. Res. Commun.1995; 206:764-74. |
| Immunogen | BRCC45 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC45. The immunogen is located within the last 50 amino acids of BRCC45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRE |
|---|---|
| Alternative Names | BRCC45 Antibody: BRCC4 BRCC45 BRCA1-A complex subunit BRE Brain and reproductive organ-expressed protein |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of BRCC45 in human brain tissue with BRCC45 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BRCC45 in human brain tissue with BRCC45 antibody at 20 μg/mL.
Application
Western blot analysis of BRCC45 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with BRCC45 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BRCC45 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BRCC45 Antibody is supplied in PBS containing 0.02% sodium azide. |
