BRD2 encodes a mitogen-activated kinase which localizes to the nucleus. BRD2 maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3 but sequence comparison suggests that the protein is not involved in the immune response. Homology to the Drosophila gene female sterile homeotic suggests that this human gene may be part of a signal transduction pathway involved in growth control.1) Pal, D.K., et al., (2003) Am. J. Hum. Genet. 73 (2), 261-270.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
BRD2 antibody can be used for detection of BRD2 by ELISA at 1:62500. BRD2 antibody can be used for detection of BRD2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
BRD2 antibody can be used for detection of BRD2 by ELISA at 1:62500. BRD2 antibody can be used for detection of BRD2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRD2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.