This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. BRD3 is ubiquitously expressed in human adult and fetal tissues. BRD3 is markedly expressed in undifferentiated ES cells, whereas the expression was reduced upon endothelial differentiation. BRD3 plays a role in regulation of cell proliferation by allowing cells to enter the proliferative phase of the angiogenic process.BRD3 is also involved in bladder cancer.1) Ishii, H., (2005) DNA Cell Biol. 24 (7), 432-437.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
BRD3 antibody can be used for detection of BRD3 by ELISA at 1:312500. BRD3 antibody can be used for detection of BRD3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
BRD3 antibody can be used for detection of BRD3 by ELISA at 1:312500. BRD3 antibody can be used for detection of BRD3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRD3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt