Application
Antibody used in CHIP on HCT116 Cells.Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.| Product Name | BRD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRD4, CAP, HUNKI, MCAP, HUNK1 |
| Product description | BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t (15;19) (q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.1) Schweiger, M.R., (2006) J. Virol. 80 (9), 4276-4285. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BRD4 antibody can be used for detection of BRD4 by ELISA at 1:1562500. BRD4 antibody can be used for detection of BRD4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRD4 |
|---|---|
| Alternative Names | BRD4 CAP HUNKI MCAP HUNK1 |
| Molecular Weight(MW) | 80 kDa, 152 kDa |
Application
Antibody used in CHIP on HCT116 Cells.
Application
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate |
|---|---|
| Application Notes | BRD4 antibody can be used for detection of BRD4 by ELISA at 1:1562500. BRD4 antibody can be used for detection of BRD4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM272702