Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Proteasome 20S beta 5 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PSMB5 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PSMB5 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMB5 (proteasome (prosome, macropain) subunit, beta type, 5)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PSMB5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LMPX antibody MB1 antibody MGC104214 antibody X antibody PSMB5 antibody "proteasome subunit beta type 5 antibody" macropain epsilon chain antibody proteasome subunit MB1 antibody proteasome subunit X antibody proteasome beta 5 subunit antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Proteasome 20S beta 5 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PSMB5 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
