Application
Western blot analysis of extracts of various cell lines, using PSMB8 antibody.Application
Immunohistochemistry of paraffin-embedded rat heart using PSMB8 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human colon using PSMB8 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human kidney using PSMB8 antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of A549 cell using PSMB8 antibody. Blue: DAPI for nuclear staining.| Product Name | PSMB8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMB8The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative t |
| Immunogen | Recombinant protein of human PSMB8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D6S216E RING10 ALDD LMP7 D6S216 JMP proteasome subunit beta 8 PSMB5i NKJO |
|---|---|
| Target | PSMB8 |
Application
Western blot analysis of extracts of various cell lines, using PSMB8 antibody.
Application
Immunohistochemistry of paraffin-embedded rat heart using PSMB8 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human colon using PSMB8 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human kidney using PSMB8 antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of A549 cell using PSMB8 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
