Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the detected BRWD2 protein (arrow).| Product Name | BRWD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WD repeat-containing protein 11, Bromodomain and WD repeat-containing protein 2, WD repeat-containing protein 15, WDR11, BRWD2, KIAA1351, WDR15 |
| Product description | WDR11 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus.1) Katoh, M., et al. Int. J. Mol. Med. 11(5):579-583(2003) |
| Immunogen | This BRWD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-572 amino acids from the Central region of human BRWD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR11 |
|---|---|
| Alternative Names | WD repeat-containing protein 11 Bromodomain and WD repeat-containing protein 2 WD repeat-containing protein 15 WDR11 BRWD2 KIAA1351 WDR15 |
| Molecular Weight(MW) | 137 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in mouse testis tissue lysates (15ug/lane).This demonstrates the detected BRWD2 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
