Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Ab-700).Application
Western blot analysis of lysed extracts from HeLa and HT29 cells using c-Cbl (Ab-700).| Product Name | c Cbl Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBL2, NSLL, C-CBL, RNF55, FRA11B, CSDD2, Signal transduction protein CBL, Proto-oncogene c-CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, |
| Product description | Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.1) Blake, T. J. et al. (1991) Oncogene 6, 653-657. Thien, C. B. and Langdon, W. Y. (1998) Immunol. Cell Biol. 76, 473-482. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339. Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106. |
| Immunogen | c-Cbl (Ab-700) antibody was raised against a peptide sequence around aa. 698~702 (T-E-Y-M-T) derived from Human c-Cbl. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBL |
|---|---|
| Alternative Names | CBL2 NSLL C-CBL RNF55 FRA11B CSDD2 Signal transduction protein CBL Proto-oncogene c-CBL Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 |
| Molecular Weight(MW) | 120 kDa |
| Tissue Specificity | This antibody detects endogenous level of total c-Cbl protein. |
| Entrez Gene | 867 |
|---|---|
| Protein Accession | NP_005179.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Ab-700).
Application
Western blot analysis of lysed extracts from HeLa and HT29 cells using c-Cbl (Ab-700).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
