Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using c-Cbl (phospho-Tyr700).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Phospho-Tyr700). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using c-Cbl (phospho-Tyr700).| Product Name | c Cbl (phospho Tyr700) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBL2, NSLL, C-CBL, RNF55, FRA11B, NR3C3, Signal transduction protein CBL, Proto-oncogene c-CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, |
| Product description | Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.1) Blake, T. J. et al. (1991) Oncogene 6, 653-657. Thien, C. B. and Langdon, W. Y. (1998) Immunol. Cell Biol. 76, 473-482. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339. Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106. |
| Immunogen | c-Cbl (phospho-Tyr700) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y (p) -M-T) derived from Human c-Cbl. |
| Modification | p-Tyr700 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBL |
|---|---|
| Alternative Names | CBL2 NSLL C-CBL RNF55 FRA11B NR3C3 Signal transduction protein CBL Proto-oncogene c-CBL Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 |
| Molecular Weight(MW) | 120 kDa |
| Tissue Specificity | This antibody detects endogenous level of c-Cbl only when phosphorylated at tyrosine 770. |
| Entrez Gene | 867 |
|---|---|
| Protein Accession | NP_005179.2 |
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using c-Cbl (phospho-Tyr700).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Phospho-Tyr700). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using c-Cbl (phospho-Tyr700).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
