Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Ser73).Application
Western blot analysis of lysed extracts from PC14 cells untreated or treated with EGF using c-Jun (Phospho-Ser73).| Product Name | c Jun (phospho Ser73) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP1, AP-1, c-Jun, MEK2, MKK2, PRKMK2, AH119, AP1, Jun A, p39 |
| Product description | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.1) Sevilla A, et al. (2004) Oncogene. 23 (55): 8950-8958. Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Binetruy B, et al. (1991) Nature. 351:122-127. Smeal T, et al. (1991) Nature. 354: 494-496. Derijard B, et al. (1994) Cell. 76:1025-1037. |
| Immunogen | c-Jun (Phospho-Ser73) antibody was raised against a peptide sequence around phosphorylation site of serine 73 (L-A-S (p) -P-E) derived from Human c-Jun. |
| Modification | p-Ser73 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | JUN |
|---|---|
| Alternative Names | AP1 AP-1 c-Jun MEK2 MKK2 PRKMK2 AH119 AP1 Jun A p39 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of c-Jun only when phosphorylated at serine 73. |
| Entrez Gene | 3725 |
|---|---|
| Protein Accession | NP_002219.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Ser73).
Application
Western blot analysis of lysed extracts from PC14 cells untreated or treated with EGF using c-Jun (Phospho-Ser73).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
