Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit (Ab-936).Application
Western blot analysis of lysed extracts from HL60 cells using c-kit (Ab-936).| Product Name | c kit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PBT, SCFR, C-Kit, CD117, HSP27, HSP28, KIT, PBT |
| Product description | This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)1) Martin, F. H. et al. (1990) Cell 63, 203-11. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62. Gommerman, J. L. et al. (1997) J Biol Chem 272, 30519-25. Nocka, K. et al. (1990) EMBO J 9, 1805-13. |
| Immunogen | c-kit (Ab-936) antibody was raised against a peptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KIT |
|---|---|
| Alternative Names | PBT SCFR C-Kit CD117 HSP27 HSP28 KIT PBT |
| Molecular Weight(MW) | 145 kDa |
| Tissue Specificity | This antibody detects endogenous level of total c-Kit protein. |
| Entrez Gene | 3815 |
|---|---|
| Protein Accession | NP_000213.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit (Ab-936).
Application
Western blot analysis of lysed extracts from HL60 cells using c-kit (Ab-936).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
