Application
Western blot analysis of anti-C-rel (NFkB) Pab (AP6336c)in Jurkat (left) and mouse kidney (right) cell line lysate.Application
Western blot analysis of lysate from Daudi cell line, using C-rel (NFkB)-G601 Antibody at 1:1000 at each lane.| Product Name | C-rel Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proto-oncogene c-Rel, REL |
| Product description | Nuclear factor (NF)-kappa B is a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB regulates the expression of a wide variety of genes that involved in apoptosis, viral life cycle, tumorigenesis, autoimmune diseases and inflammation. NF-kB is a heterodimer of members of the rel family of proteins such as p50, p65, and c-rel. In most cells, inhibitory IkB proteins sequester NF-kB/Rel in the cytoplasm. Cellular stimulation precipitates degradation of IkB and modification of NF-kB/Rel proteins, permitting translocation of NF-kB/Rel (c-Rel and RelA) to the nucleus for action on target genes. The important role of c-Rel in B-cell development, growth, and survival has been intensively studied, as well as its function in differentiation and lymphopoiesis (particularly lymphoid cancer).1) Jain, A., et al., J. Clin. Invest. 114(11):1593-1602 (2004). |
| Immunogen | This C-rel (NFkB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-619 amino acids from the C-terminal region of human C-rel (NFkB). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REL |
|---|---|
| Alternative Names | Proto-oncogene c-Rel REL |
| Molecular Weight(MW) | 69 kDa |
Application
Western blot analysis of anti-C-rel (NFkB) Pab (AP6336c)in Jurkat (left) and mouse kidney (right) cell line lysate.
Application
Western blot analysis of lysate from Daudi cell line, using C-rel (NFkB)-G601 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
