Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | C/EBP-alpha (phospho Ser21) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAP4, ICE8, MACH, MCH5, Mch-5 |
| Product description | C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.1) Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461. |
| Immunogen | C/EBP-alpha (phospho Ser21) antibody was raised against a peptide sequence around phosphorylation site of Serine 21 (L-Q-S (p)-P-P) derived from Human C/EBP-alpha. |
| Modification | p-Ser21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CEBPA |
|---|---|
| Alternative Names | CAP4 ICE8 MACH MCH5 Mch-5 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | The antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at serine 21. |
| Entrez Gene | 1050 |
|---|---|
| Protein Accession | NP_004355.2 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-alpha (phospho-Ser21) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-alpha (phospho-Ser21) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
