Application
Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-alpha (Phospho-Thr230) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | C/EBP-alpha (phospho Thr230) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA |
| Product description | The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.1) Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995) |
| Immunogen | C/EBP-alpha (phospho Thr230) antibody was raised against a peptide sequence around phosphorylation site of threonine 230 (P-P-T (p)-P-V) derived from Human C/EBP-alpha. |
| Modification | p-Thr230 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CEBPA |
|---|---|
| Alternative Names | CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA |
| Molecular Weight(MW) | 42 kDa |
| Tissue Specificity | The Antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at Thr230. |
| Entrez Gene | 1050 |
|---|---|
| Protein Accession | NP_004355.2 |
Application
Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-alpha (Phospho-Thr230) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
