Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG 10% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PSMD12 antibody at 1:500 dilution.| Product Name | PSMD12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMD12 (proteasome (prosome, macropain) 26S subunit, non-ATPase, 12)The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (99%), Rat (99%), Zebrafish (84%), Xenopus laevis (92%), Chicken (93%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC75406 antibody Rpn5 antibody p55 antibody PSMD12 antibody 26S proteasome regulatory subunit RPN5 antibody 26S proteasome regulatory subunit p55 antibody 26S proteasome non-ATPase regulatory subunit 12 antibody "proteasome (prosome macropain) 26S |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG 10% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PSMD12 antibody at 1:500 dilution.| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
