Application
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | C4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This C4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 655-684 amino acids from the Central region of human C4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C4A |
|---|---|
| Alternative Names | Complement C4-A Acidic complement C4 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 Complement C4 beta chain Complement C4-A alpha chain C4a anaphylatoxin C4b-A C4d-A Complement C4 gamma chain C4A CO4 CPAMD2 |
| Molecular Weight(MW) | 193 kDa |
Application
Western Blot at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
